Whole genome amplification using a degenerate oligonucleotide primer allows hundreds of genotypes to be performed on les...

Cheung, Vg Stanley F. Nelson

Published in Proceedings of the National Academy of Sciences

Genetic analysis of limiting quantities of genomic DNA play an important role in DNA forensics, paleoarcheology, genetic disease diagnosis, genetic linkage analysis, and genetic diversity studies. We have tested the ability of degenerate oligonucleotide primed polymerase chain reaction (DOP-PCR) to amplify picogram quantities of human genomic DNA f...

Identification of allele-specific alternative mRNA processing via transcriptome sequencing.

Li, G Bahn, Jh Lee, Jh Peng, G Chen, Z Stanley F. Nelson Xiao, X

Published in Nucleic Acids Research

Establishing the functional roles of genetic variants remains a significant challenge in the post-genomic era. Here, we present a method, allele-specific alternative mRNA processing (ASARP), to identify genetically influenced mRNA processing events using transcriptome sequencing (RNA-Seq) data. The method examines RNA-Seq data at both single-nucleo...